Suitable for use in Flow Cytometry and Immunohistochemistry (paraffin-embedded sections). Other applications not tested.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|Purified by Protein G affinity chromatography.|
|50% Glycerol, PBS|
|Detects the CD11b antigen (mW170 and 95kD) also known as Mac-1 antigen, found on mouse macrophages, their precursors and granulocytes. Species Crossreactivity: Crossreacts with Human, Mouse and Rabbit. |
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
This product has been used in:Whiteland JL et al. Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem 43:313-20 (1995). <7868861>Smet EG et al. Mab. 198: a monoclonal antibody recognizing the complement type 3 receptor (CR3) in the rabbit. Immunology 59:419-25 (1986). <3793137>Lopez AF et al. Epitope diversity of monoclonal antibodies revealed by cross-species reactivity. Mol Immunol 21:371-4 (1984). <6204196>Ho MK & Springer TA Mac-1 antigen: quantitative expression in macrophage populations and tissues, and immunofluorescent localization in spleen. J Immunol 128:2281-6 (1982). <7037963>Kurzinger K & Springer TA Purification and structural characterization of LFA-1, a lymphocyte function-associated antigen, and Mac-1, a related macrophage differentiation antigen associated with the type three complement receptor. J Biol Chem 257:12412-8 (1982). <6749860>Ault KA & Springer TA Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells. J Immunol 126:359-64 (1981). <7451976>Springer T et al. Mac-1: a macrophage differentiation antigen identified by monoclonal antibody. Eur J Immunol 9:301-6 (1979). <89034>Springer T et al. Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur J Immunol 8:539-51 (1978). <81133> Milstein C et al. Monoclonal antibodies and cell surface antigens. Ciba Found Symp :251-76 (1979). <258170>|