Description:
Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry (paraffin-embedded sections). Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG2b | 8J585 | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 100ug | | -20°C | Blue Ice | | Rat |
|
| Concentration: |
| ~1mg/ml |
| Immunogen: |
|
| Purity: |
| Purified by Protein G affinity chromatography. |
| Form |
| 50% Glycerol, PBS |
| Specificity: |
| Detects the CD11b antigen (mW170 and 95kD) also known as Mac-1 antigen, found on mouse macrophages, their precursors and granulocytes. Species Crossreactivity: Crossreacts with Human, Mouse and Rabbit. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
This product has been used in:Whiteland JL et al. Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem 43:313-20 (1995). Smet EG et al. Mab. 198: a monoclonal antibody recognizing the complement type 3 receptor (CR3) in the rabbit. Immunology 59:419-25 (1986). Lopez AF et al. Epitope diversity of monoclonal antibodies revealed by cross-species reactivity. Mol Immunol 21:371-4 (1984). Ho MK & Springer TA Mac-1 antigen: quantitative expression in macrophage populations and tissues, and immunofluorescent localization in spleen. J Immunol 128:2281-6 (1982). Kurzinger K & Springer TA Purification and structural characterization of LFA-1, a lymphocyte function-associated antigen, and Mac-1, a related macrophage differentiation antigen associated with the type three complement receptor. J Biol Chem 257:12412-8 (1982). Ault KA & Springer TA Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells. J Immunol 126:359-64 (1981). Springer T et al. Mac-1: a macrophage differentiation antigen identified by monoclonal antibody. Eur J Immunol 9:301-6 (1979). Springer T et al. Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur J Immunol 8:539-51 (1978). Milstein C et al. Monoclonal antibodies and cell surface antigens. Ciba Found Symp :251-76 (1979).
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