Technical Data
C2262-39J
CD11B (Integrin alpha-M, CD11 Antigen-like Family Member B, CR-3 alpha Chain, Cell Surface Glycoprotein MAC-1 Subunit alpha, Leukocyte Adhesion Receptor MO1, Neutrophil Adherence Receptor, ITGAM, CR3A)
Description:
Leukocyte associated protein consists of two polypeptide chains with molecular weights of 95 and 170kD. The molecule functions as a cell surface receptor for the C3bi complement fragment. It belongs to family of heterodimeric proteins (designated CD11), which share a common chain CD18 of 95kD. The chain component of the leukocyte- associated antigen is expressed on granulocytes, monocytes and macrophages.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:50 for 30min at RT
Staining of formalin-fixed tissues requires boiling tissues sections in 10mM citrate buffer, pH 6.0, for 10min followed by cooling at RT for 20min
Optimal dilutions to be determined by the researcher.

Positive Control:
Jurkat cells. Tonsil

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~0.1mg/ml
Immunogen:
Recombinant correspondingg to aa936-1154 from human CD11b.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
Specificity:
Recognizes human CD11b.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.