Technical Data
C2262-45
CD11b (Mac-1, alphaM integrin, Cr3, MO-1, C3bi Receptor) (Biotin)
Description:
The CD11b/CD18 antigen belongs to the beta2 intergrin family. Other names include Mac-1, Cr3, MO-1 and C3bi receptor. The CD11b molecule functions as a receptor for C3bi complement, clotting factor X, brinogen and ICAM-1. The CD11b antigen is expressed on approximately 30% of human peripheral blood lymphocytes, most natural killer lymphocytes, circulating monocytes, granulocytes, a subset of T-cells and subsets of macrophages (1,2).

Applications:
Suitable for use in Flow Cytometry and in Cell adhesion research. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1ug to stain 1x10e6 cells.
Optimal dilutions to be determined by the researcher.

Storage and Stability:

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG11.BB.187Affinity Purified
SizeStorageShippingSourceHost
200ug4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Synovial cells and human momocytes.
Purity:
Purified by Protein G chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide. Labeled with Biotin.
Specificity:
Recognizes the alpha subunit of the CD11b/CD18 complex, an alpha/Beta heterodimeric surface glycoprotein on human leukocytes (Mr 165-170kD).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Myones, B.L. et al (1988) J. Clin. Invest. 82:640. 2. Malhotra, V. et al (1986) Eur. J. Immunol. 16:1117. 3. Leucocyte Typing: 2nd Workshop: Code No. M104 3rd Workshop: Code No. 202 and 238. 4th Workshop: Code No. M147.