		Technical Data
C2262-47	CD11b (Mac-1, alphaM integrin, Cr3, MO-1, C3bi Receptor) (FITC)

Description:
CD11b antigen is expressed on approximately 30% of human peripheral blood lymphocytes, most natural killer lymphocytes, circulating monocytes, granulocytes, a subset of T-cells and subsets of macrophages. CD11b/CD18 antigen belongs to the beta-2 integrin family. Other names include Mac-1, Cr3, MO-1 and C3bi receptor. The CD11b molecule functions as a receptor for C3bi complement, clotting factor X, brinogen and ICAM-1.

Applications:
Suitable for use in Immunohistology (acetone fixed or frozen sections), cell adhesion research, and Flow cytometry. Other applications have not been tested.

Recommended Dilution:
Flow Cytometry: 1ug to stain1x10e6 cells
Optimal dilutions to be determined by the researcher.

Absorption wavelength:
492nm

Emission wavelength:
518nm

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	1.BB.187	Affinity Purified

Size		Storage	Shipping	Source	Host
200ug		4°C (-20°C Glycerol)	Blue Ice	Human	Mouse

Concentration:

~0.1mg/ml

Immunogen:

Synovial cells and human monocytes.

Purity:

Purified by affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with FITC.

Specificity:

Recognizes the alpha subunit of the CD11b/CD18 complex, an alpha/beta heterodimeric surface glycoprotein on human leukocytes, Mr 165–170kD.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Myones, B.L. et al (1988) J. Clin. Invest. 82:640. 2. Malhotra, V. et al (1986) Eur. J. Immunol. 16:1117. 3. Leucocyte Typing: 2nd Workshop: Code No. M104 3rd Workshop: Code No. 202 and 238. 4th Workshop: Code No. M147.