		Technical Data
C2262-52	CD11b (Mac-1, alphaM integrin, Cr3, MO-1, C3bi Receptor)

Description:
Source: Murine ascites

Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence, Flow Cytometry: 2ug/5x10e5 cells/test.
Immunohistochemistry: 1:25 to 1:50. Only suitable on cryostat sections or cell smears.

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	8.F.37	Purified

Size		Storage	Shipping	Source	Host
200ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Normal human blood granulocytes.

Purity:

Purified by column chromatography.

Form

Supplied as a liquid in PBS buffer, pH 7.5, 0.08% sodium azide.

Specificity:

Highly reactive with CD11b antigen (MW 165kD).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.