Technical Data
C2262-55E
CD11c (Integrin alpha-X, gp150/95 subunit)
Description:
CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue.

Applications:
Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

TypeIsotypeCloneGrade
MabIgG18J588Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Tissue / cell preparation (Human). Synovial cells and human monocytes. Cellular Localization: Type I membrane protein.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 1mg/ml BSA.
Specificity:
Recognizes the X chain of the human CD11c/CD18 (p150/95) integrin heterodimer. Species Crossreactivity: Rhesus monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Arnaout MA Leukocyte adhesion molecules deficiency: its structural basis, pathophysiology and implications for modulating the inflammatory response. Immunol Rev 114:145-80 (1990). 2. Patarroyo M et al. Leukocyte-cell adhesion: a molecular process fundamental in leukocyte physiology. Immunol Rev 114:67-108 (1990). 3. Kishimoto TK et al. The leukocyte integrins. Adv Immunol 46:149-82 (1989). 4. Hogg N et al. The p150,95 molecule is a marker of human mononuclear phagocytes: comparison with expression of class II molecules. Eur J Immunol 16:240-8 (1986).