		Technical Data
C2262-57D2	CD11c (Monocyte, Macrophage, Integrin alphaX) (FITC)

Description:
CD11c antigen is expressed mainly on monocytes and in low density on granulocytes and NK cells. High levels of CD11c are expressed on tissue macrophages. It is also found on lymphoid cell lines including hairy cell leukemia. CD11c (integrin aX subunit) combines with CD18 (integrin b2 subunit) to form the integrin protein 150,95 (aXb2, CD11c/CD18). CD11c/CD18 has been reported to play an important role in cytotoxic T cell killing, and in neutrophil and monocyte adhesion to endothelium. Although its ligands in these two cases have not been identified. CD11c/CD18 is not expressed on leukocytes of patients with leukocyte adhesion deficiency. leukocyte adhesion deficiency results from defects in the CD18 gene, leading to diminished expression of all CD18-containing integrins. CD11c antibodies bind multiple ligands including the complement fragment iC3b, CD54 (ICAM-1), fibrinogen and bacterial lippolysaccharide.

Applications:
Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:100. Use 10ul of the suggested working dilution to label 1x10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Spleen cells from immunized Armenian Hamster were fused with cells of the Sp2/0 myeloma cell line.

Source:
Tissue culture supernatant

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

Type	Isotype	Clone	Grade
Mab	IgG	N418	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Mouse	Hamster

Concentration:

~0.1mg/ml

Immunogen:

Mouse spleen dendritic cells.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).

Specificity:

Recognizes the murine homologue of human CD11c. Has been reported to enhance antigen specific responses when used to target dendritic cells in vivo (3).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Crowley, M.T., et al., Use of fluorescence activated cell sorter to enrich dendritic cells from mouse spleen. J. Immunol. Methods 133: 55 (1990). 2. Metlay, J.P., et al., The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies. J. Exp. Med. 171: 1753 (1990). 3. Wang, H., et al., Rapid antibody responses by low-dose, single-step dendritic cell-targeted immunization. PNAS 97: 847-852 (2000).