Technical Data
C2264-14B
CD13 (Myeloid Cells, Aminopeptidase N)
Description:
Aminopeptidase N (APN) is a cell surface protein homologous with human CD13. In the mouse, CD13 is a non-covalently linked homodimer of approximately 150kD subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. This clone is reported to block mouse APN enzyme activity. C2264-14F (azide free) is recommended for blocking. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50-1:100. Use 10ul to label 10e6 cells in 100ul.
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.

Hybridoma:
Spleen cells from immunized mice were fused with cells of the IR983F rat myeloma cell line.

Recommended Negative Control:
I1904-82D1: Rat IgG2a Negative Control

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a3H1010Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRat
Concentration:
~1mg/ml
Immunogen:
Mouse intestinal APN
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes mouse APN.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hansen, A.S., et al., Eur. J. Immunol. 23: 2358-2364 (1993). 2. Larsen, S.L., et al., J. Exp. Med. 184: 183-189 (1996). 3. Kamoun, W.S., et al., J. Clin. Oncol. 27: 2542-2552 (2009).