		Technical Data
C2264-14B	CD13 (Myeloid Cells, Aminopeptidase N)

Description:
Aminopeptidase N (APN) is a cell surface protein homologous with human CD13. In the mouse, CD13 is a non-covalently linked homodimer of approximately 150kD subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. This clone is reported to block mouse APN enzyme activity. C2264-14F (azide free) is recommended for blocking. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50-1:100. Use 10ul to label 10e6 cells in 100ul.
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.

Hybridoma:
Spleen cells from immunized mice were fused with cells of the IR983F rat myeloma cell line.

Recommended Negative Control:
I1904-82D1: Rat IgG2a Negative Control

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a	3H1010	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Mouse	Rat

Concentration:

~1mg/ml

Immunogen:

Mouse intestinal APN

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

Specificity:

Recognizes mouse APN.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Hansen, A.S., et al., Eur. J. Immunol. 23: 2358-2364 (1993). 2. Larsen, S.L., et al., J. Exp. Med. 184: 183-189 (1996). 3. Kamoun, W.S., et al., J. Clin. Oncol. 27: 2542-2552 (2009).