Technical Data
C2266-20J
CD15 (Alpha-(1,3)-fucosyltransferase, ELAM-1 Ligand Fucosyltransferase, ELFT, FCT3A, Fucosyltransferase 4, FUT4, Fucosyltransferase IV, Fuc-TIV, FucT-IV, FUTIV, Galactoside 3-L-fucosyltransferase, LeX, SSEA-1)
Description:
CD15 is found on most circulating granulocytes and some monocytes, but is absent from normal lymphocytes. It has been reported to be present on Reed- Sternberg cells of Hodgkin’s disease, T cell lymphomas and some leukaemias. This antibody will help detect CD15 expression on normal and neoplastic cells.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:20 for 1 hr. at RT
Optimal dilutions to be determined by the researcher.

Positive Control:
Small intestine, membrane staining pattern and granular paranuclear labeling seen in some cells

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgMBY87Supernatant
SizeStorageShippingSourceHost
1ml-20°CBlue IceMouse
Concentration:
Not determined
Immunogen:
Peripheral blood cells from a patient with B cell lymphoma.
Purity:
Supernatant
Form
Supplied as a liquid in 15mM sodium azide.
Specificity:
Recognizes CD15. Species Crossreactivity: Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Hall P A and D’Ardenne A J. Value of CD15 immunostaining in diagnosing Hodgkin’s disease: a review of the published literature. Journal of Clinical Pathology. 40:1298-1304 (1987). 2.Lee F D. Hodgkin’s disease. Histopathology. 11:1211-1217 (1987). 3.Dorfman R F, Gatter K C, Pulford K A, et al.. An evaluation of the utility of anti-granulocyte and anti-leukocyte monoclonal antibodies in the diagnosis of Hodgkin’s disease. American Journal of Pathology. 123:508-519 (1986).