		Technical Data
C2269-51	CD19

Description:
CD19 (B-lymphocyte antigen CD19 or B- lymphocyte surface antigen B4) is a cell surface molecule that assembles with the antigen receptor of B lymphocytes (CD21, CD81 and CD225) and decreases the threshold for antigen receptor-dependent stimulation. Lymphocytes proliferate and differentiate in response to various antigens. CD19 is expressed on follicular dendritic cells and B cells. CD19 is present on B cells from the earliest recognizable B-lineage cells during development to B-cell blasts, but is lost on maturation to plasma cells. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated on thyrosine. Serine and threonine phosphorylation of CD19 occurs upon DNA damage. Defects in CD19 are a cause of hypogammaglobulinemia.
The CD19 antigen (90 kDa) is expressed from the earliest stages of B-progenitor development, on all peripheral B cells including germinal centre B cells and all B cell lines and B cell leukaemias tested. T cell and monocytic cell lines are negative and the antigen is lost on B cell maturations to plasma cells. The antigen is a type I integral membrane glycoprotein whose in vitro inhibition will influence B cell activation and proliferation.

Applications:
Suitable for use in Indirect Immunofluorescence, Immunoprecipitation, Flow Cytometry, Immunohistochemistry, Leukemia and lymphoma phenotyping and Studies of the CD19/21 complex in B cell activation and the control of B cell development. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Identifies normal and malignant CD19 positive B lymphocytes in cryostat frozen sections.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	2Q1257	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.1mg/ml

Immunogen:

Hairy cell leukemia cells

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, 1mg/ml BSA, 10mM sodium azide.

Specificity:

Recognizes human CD19.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Carlisle, M., et al. (2003). International Immunology. 15:17-27.2. Riedl, E., et al. (2000). Blood. 96:4276-4284. 3. Golay, J., et al. (2000). Blood. 95:3900-3908. 4. Karp, D., et al. (1999). InternationalImmunology. 11:1791-1800. 5. Strobl, H., et al. (1998). J. Immunology.161:740-748. 6. Fischer, G., et al. (1990). J. Immunology.144:638-641.