Technical Data
C2269-51E1
CD19
Description:
CD19 is a type I transmembrane glycoprotein important in B-lymphocyte signal transduction.1 Together with CD21, CD81, and beta 1 integrin, it is a part of the B-lymphocyte signaling complex, which regulates B-cell activation and differentiation.2 Originally identified as B4, CD19 is a pan B-cell antigen expressed from the B-cell progenitor stage where rearrangement of immunoglobulin genes takes place, to the terminal stage of B-cell differentiation into plasma cells. CD19 is detected on follicular dendritic cells and malignant B-cells.

Applications:
Suitable for use in Immunocytochemistry, Immunohistochemistry and Flow Cytometry. Other applications have not been tested.

Recommended Dilutions:
Immunocytochemistry: 2-10ug/ml
Immunohistochemistry (Frozen): 2-10ug/ml
Optimal conditions should be determined individually for each application.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG16D255Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human CD19
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH7.2, 0.02% sodium azide.
Specificity:
Recognizes human CD19. Identifies human leukemias and lymphomas.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Isolation of cDNAs encoding the CD19 antigen of human and mouse B lymphocytes. A new member of the immunoglobulin superfamily: T.F. Tedder, et al.; J. Immunol. 143, 712 (1989) 2. The CD19 signal transduction molecule is a response regulator of B-lymphocyte differentiation: S. Sato, et al.; PNAS 92, 11558 (1995) 3. CD19 monoclonal antibody HD37 inhibits anti-immunoglobulin-induced B cell activation and proliferation: A. Pezzutto, et al.; J. Immunol. 138, 2793 (1987) 4. B4, a human B lymphocyte-associated antigen expressed on normal, mitogen-activated, and malignant B lymphocytes: L.M. Nadler, et al.; J. Immunol. 131, 244 (1983)