Technical Data
C2274-02N
CD23 (CD23A, BLAST-2, C-type Lectin Domain Family 4 Member J, CLEC4J, Fc-epsilon-RII, FCE2, FCER2, Immunoglobulin E-binding Factor, IGEBF, Low Affinity Immunoglobulin epsilon Fc Receptor, Lymphocyte IgE Receptor) (FITC)
Description:
Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: frozen
Flow Cytometry: 5ul labels 10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG1Tü 1Purified
SizeStorageShippingSourceHost
500ul-20°CBlue IceMouse
Concentration:
Not determined.
Immunogen:
CD23, B cell.
Purity:
Purified
Form
Supplied as a liquid in BSA, sodium azide. Labeled with FITC.
Specificity:
Recognizes CD23, B cell, at ~45-50kD. Also reacts with a subpopulation of B-lymphocytes in peripheral blood and also in frozen sections of lymphoid tissues.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Thorley-Lawson,D.,Nadler,L.M.,Bhan,A.D. and Shooley,R.T. 1985, J.Immunol.134: 3007. 2. Kikutani, H., Inui, S. ,Sato, R., Barsumian, E.L., Owaki, H., Yamasaki, K., Kaisho, T., Uchibayashi, N., Hardy, R.R.Hirano, T. Tsunasawa, S., Sakiyama, F., Masaki, S. and Kishimotor, T. 1986 Cell 47:657. 3. Kikutani, H., Suemura, M., Owaki, H., Nakamura, H., Sato, R., Yamaski, K., Barumian, E.L., Hardy,R. and Kishimotor, T. 1986 J.Exp. xxxx 164:1455. 4. Swendeman, S. and Thorley-Lawson, D.A. 1987 EMBD J. 6:1637. 5. Yukawa, K., Kikutani, H., Owaki, H., Katsuhiko, Y., Yokota, A., Nakamura,H., Barumian, E.L., Hardy, R.R., Suemura, M. and Kishimoto,T. 1987 J.Immunol. 138:2576. 6. Guy, G.R. and Gordon, J. 1987 Proc.Nat.Acad.Sci.LISA 84: 6239. 7. Péne J., Rousset, F., Briére, F., Chrétién, I., Wideman,J., Bonnefoy, J.Y., and de Vries,J.E.1988 Eur.J. Immunol. 18:929. 8. Vercelli, D., Jabara, H.H., Lee, B.W., Woodland, N., Geha, and Leung, D.Y.M. 1988 J.Exp.Med. 16:1406.