Technical Data
C2275-07
CD24 (GPI-linked surface Mucin, Nectadrin)
Description:
CD24 is a single chain glycosylphosphatidylinositol (GPI)-anchored cell surface sialoglycoprotein. Expressed early in B lymphocytes ontogeny and lost at the plasma cell stage. In contrast, CD24 antigen is absent from T lymphocytes, NK cells, monocytes, erythrocytes and platelets.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunocytochemistry and studies of peripheral blood B cells. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: Frozen sections
Immunocytochemistry: Cell smears
Optimal dilutions to be determined by the researcher.

Hybridoma:
NS1/AG4.1 myeloma cells with spleen cells from Balb/c mice.

Source: Ascites

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
TypeIsotypeCloneGrade
MabIgG18.B.76Purified
SizeStorageShippingSourceHost
200ug-20°CBlue IceHumanMouse
Concentration:
~0.2mg/ml (after reconstitution)
Immunogen:
Human bone marrow malignant cells from a non-B, non-T acute leukemia.
Purity:
Purified
Form
Supplied as a lyophilized powder from PBS, 1mg/ml BSA. Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes human CD24 at 35-45kD. Recognizes B cells from pre-B stage to mature-B stage.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Boucheix, C., et al., Leuk. Res., 9 (5):597–604 (1985) 2. Duperray, C., et al., J. Immunol, 145 (11):3678–3683 (1990) 3 .Weber E,et al., . Clin Exp Immunol. Aug;93(2):279-85. (1993). 4. Chappel, S., Kay, R., Humphries, R.K. “CD24 Workshop Panel report”, 1997, Leucocyte Typing VI, White Cell Differentiation Antigens, Kishimoto, T., et. al., Eds., Garland Publishing, Inc., 147–149.