Technical Data
C2383-02H
CD31 (PECAM-1, Platelet gpIIa Molecule)
Description:
PECAM-1 is a member of the immunoglobulin superfamily. This heavily glycosylated protein is found in the entire vascular endothelium of adult mice and functions in mediating cellular adhesion by heterophilic and homophilic mechanisms. Reacts with the major population of colony forming unit macrophage (CFU-M) precursor cells, with a subpopulation of pre CFU-M and monoblasts, and with precursor cells of granulocytes. PECAM-1 is detected within the lymphopoietic islands in the spleen of newborn (day 12) and in the bone marrow of adult mice. Capillary endothelial cells of adult mice also express PECAM-1. The monoclonal antibody can be used, in combination with the monoclonal antibody, for
identification of different stages of myeloid progenitor cell development.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50
Immunohistochemistry (frozen or paraffin): 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a9G146Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRat
Concentration:
~0.1mg/ml
Immunogen:
Mouse macrophage cell lines.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Specificity:
Recognizes an antigen present on cell subpopulations of adult mouse bone marrow, including pluripotent hematopoietic stem cells. The mouse hematopoietic progenitor cell antigen is Platelet-Endothelial Cell Adhesion Molecule (PECAM)-1 (CD31) of 113kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Leenen, P et al; Murine macrophage precursor characterization II. Monoclonal antibodies against macrophage precursor antigens. Eur J Immunol 1990, 20: 27 2. De Bruijn, M et al; Distinct mouse bone marrow macrophage precursors identified by differential expression of ER-MP12 and ER-MP20 antigens. Eur J Immunol 1994, 24: 2279 3. Ling, V et al; Structural identification of the hematopoeitic progenitor antigen ER-MP12 as the vascular endothelial adhesion molecule PECAM-1 (CD31). Eur J Immunol 1997, 27: 509 4. De Bruijn, M et al; Bone marrow cellular composition in Listeria monocytogenes infected mice detected using ER-MP12 and ER-MP20 antibodies: a flow cytometric alternative to differential counting. J Immunol Methods 1998, 217: 27