Technical Data
C2383-10A
CD31 (PECAM-1, Platelet gpIIa Molecule)
Description:
CD31 (PECAM-1, Platelet gpIIa Molecule), a ~130kD integral membrane glycoprotein, is expressed in platelets, cultured endothelial cells, monocytes, normal tissue macrophages in tonsil, lung and kidney, skin biopsies from conditions such as sarcoidosis and lepromatous leprosy and bone marrow-derived hematopoietic stem cells and embryonic stem cells. CD31 mediates cell-cell adhesion. Valuable in the study of benign and malignanat vaascular tumors. Stainiing for CD31 has also been used to measure angiogenesis, which reportedly predicts tumor recurrence.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence, ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:10-1:100. Use 10ul of diluted antibody to label 10e6 cells in 100ul.
Immunohistochemistry (Frozen): 1:10-1:100
Immunohistochemistry (paraffin): Staining of endothelium may be enhanced on FFPE tissue by pre-treatment with 0.2M boric acid, pH 7.0 ( see Wilson et al.)
Functional Studies: Recommend the use of C2383-03G.
Optimal working dilutions to be determined by researcher.

Hybridoma:
Sp2/myeloma cells with spleen cells from Balb/c mice.

Recommended Negative Control:
I1904-78Y: IgG1 Mouse Negative Control (rat tissues only).

Recommended Secondary Reagents:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13H1217Affinity Purified
SizeStorageShippingSourceHost
250ug-20CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Activated, Lewis rat derived microglial cells.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity:
Recognizes rat PECAM-1. Partially blocks the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen. Species Crossreactivity: Does not crossreact with human tissues.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Flaris, N.A., Densmore, T.L., Molleston, M.C. and Hickey, W.F., GLIA 7: 34-40 (1993). 2. Male, D., Rahman, J., Linke, A., Zhao, W. and Hickey, W.F., Immunology 84: 453-460 1. Flaris, N.A., et al., GLIA 7: 34-40 (1993). 2. Male, D., et al., mmunology 84: 453-460 (1995). 3. Williams, K.C., et al., J. Neurosci. Res. 45:747-757 (1996). 4. Wilson et al., J Immunol Methods 322: 137-42 (2007) 6. Stevenson, K.S. et al. (2009) Stem Cells Dev. 18: 1389-98. 7. Ott, I. et al. (2005) FASEB J. 19: 992-4. 8. Fujimoto, K.L. et al. (2007) J Am Coll Cardiol. 49: 2292-300. 9.Thebault, P. et al. (2010) J Immunol. 183: 3099-108. 10. Graham, M.J. et al. (1998)J Pharmacol Exp Ther. 286: 447-58. 11. Kielian, T. and Hickey, W.F. (2010) Am J Pathol. 157: 647-58.