Technical Data
C2383-26
CD31 (PECAM-1, Platelet gpIIa Molecule) (FITC)
Description:
CD31 (PECAM-1, Platelet gpIIa Molecule) is a 130-140kD type I intergral membrance glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. CD31 is constitutively expressed on the surface of endothelial cells where it is concentrated at cell junctoins. CD31 is also expressed at low levels on all leukocytes and platelets. Homotypic interaction of CD31 is impotant in cell-cell interaction, cell-matrix interaction and signal transduction.

Applications:
Suitable for use in Flow Cytometry, Immunoprecipitation, Immunohistochemistry, Blocking and Inhibition. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1ug labels 10e6 cells in 100ul.
Immunohistochemistry: Acetone fixation on frozen tissue.
Inhibition: Inhibits the aggregation of L cells transfected with a variant form of CD31.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG2a,k390Affinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceMouseRat
Concentration:
~0.5mg/ml
Immunogen:
Mouse leukocyte cell line 32D
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Recognizes mouse CD31 (PECAM-1), a 130-140kD type 1 integral membrane glycoprotein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Baldwin, H.S. et al. (1994). Development 120: 2539-2553. 2. DeLisser, H. et al. (1993) Curr Top Microbiol Immuno 184: 37-45.