Technical Data
CD32 (Fcg Receptor II)
CD32 mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. The antigen is expressed by B cells, monocytes, granulocytes and platelets.

Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (frozen): 1:500-1:1000
Immunoprecipitation: 20 ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Immunoprecipitation: Lymph node

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
200ug-20CBlue IceHumanMouse
Human CD32.
Supplied as a liquid in 0.09% sodium azide.
Recognizes human CD32, at ~40kD that acts as a low affinity receptor for IgG (also known as Fc gamma RII). Species Crossreactivity: canine, monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Greenman, J. et al. (1991). Characterisation of a new monoclonal anti-Fc gamma RII antibody, AT10, and its incorporation into a bispecific F(ab')2 derivative for recruitment of cytotoxic effectors. Mol. Immunol. 28:1243-1254. 2. Can den Herik-Oudjik, I.E. et al. (1994). Functional analysis of human Fc gamma RII (CD32) isoforms expressed in B lymphocytes. J. Immunol. 152:574-585. 3. Lilliehook, I. et al. (1998). Expression of adhesion and Fc gamma - receptors on canine blood eosinophils and neutophils studied by anti-human monoclonal antibodies. Vet. Immunol. Immunopathol. 61:181-193. 4. Larsson, M. et al. (1997). Human dendritic cells handling of binding, uptake and degradation of free and IgG-immune complexed dinitrophenylated human serum albumin in vitro. Immunology. 90:138-146.