The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets.
Suitable for use in Flow Cytometry. Other applications not tested.
Flow Cytometry: 10ul labels of a neat-1:10 working dilution to 10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
| As reported|
|Human CD32. |
|Supplied as a liquid in 0.09% sodium azide, 1% BSA. Labeled with FITC.|
|Recognizes human CD32 at ~40kD. Species Crossreactivity: canine, monkey. |
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Greenman, J. et al. (1991). Characterisation of a new monoclonal anti-Fc gamma RII antibody, AT10, and its incorporation into a bispecific F(ab')2 derivative for recruitment of cytotoxic effectors. Mol. Immunol. 28:1243-1254. 2. Can den Herik-Oudjik, I.E. et al. (1994). Functional analysis of human Fc gamma RII (CD32) isoforms expressed in B lymphocytes. J. Immunol. 152:574-585. 3. Lilliehook, I. et al. (1998). Expression of adhesion and Fc gamma-receptors on canine blood eosinophils and neutophils studied by anti-human monoclonal antibodies. Vet. Immunol. Immunopathol. 61:181-193. 4. Larsson, M. et al. (1997). Human dendritic cells handling of binding, uptake and degradation of free and IgG-immune complexed dinitrophenylated human serum albumin in vitro. Immunology. 90:138-146.|