Technical Data
C2386-10
CD34 (Endothelial Cell Marker, Cluster Designation 34, Hematopoietic Progenitor Cell Antigen CD34, HPCA1)
Description:
CD34, a single chain transmembrane glycoprotein, is selectively expressed on human lymphoid and myeloid hematopoietic progenitor cells. This antibody is of value in the study of benign and malignant vascular tumors as well as characterization of leukemias. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.

Applications:
Suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry (Formalin/paraffin): 1:200 for 20-60 min at RT. May require special pretreatment for histochemical staining of formalin-fixed tissues by boiling tissues in10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.

Positive Control:
Placenta

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13F85Affinity Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Detergent solubilized vesicular suspension prepared from a perfusate of human term placenta. MW of Antigen: 105-120kD Epitope: Type II epitope. Cellular Localization: Cell membrane / cytoplasmic.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C2386-10X
Specificity:
Recognizes human CD34. Also reacts with vascular endothelial cells in normal tissues and in benign and malignant proliferations. Does not react with rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ramani P; et al. Histopathology (1990) 17:237-42