Technical Data
C2386-12A
CD34 (Endothelial Cell Marker, Cluster Designation 34, Hematopoietic Progenitor Cell Antigen CD34, HPCA1)
Description:
CD34 is a 110-120kD type I transmembrane glycoprotein that belongs to the CD34/Podocalyxin family of sialomucins. It is a widely used marker of activated hematopoietic stem/progenitor cells and is also expressed on adult vascular endothelial cells. Mature rat CD34 is predicted to be a 352aa protein with a 257 aa extracellular domain (ECD) (aa35-291) and 74aa cytoplasmic tail (aa313-386). Over the region used as immunogen, rat CD34 shares 83% and 57% aa identity with mouse and human CD34, respectively.

Applications:
Suitable for use in Immunohistochemistry, Western Blot and Direct ELISA. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: 5-15ug/ml stains perfusion fixed frozen sections of rat liver.
Western Blot: 0.1ug/ml stains recombinant rat CD34.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatGoat
Concentration:
~0.2mg/ml (after reconstitution)
Immunogen:
Recombinant protein corresponding to aa37-293 from rat CD34 expressed in NS0 cells (NP_001100672). Species Sequence Homology: mouse, 83%; human, 57%
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity:
Recognizes rat CD34. Shows <1% crossreactivity with recombinant porcine CD34.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Covington JD, Fitch M, Hellerstein MK, Henagan TM, Ravussin E, Rogers PM, Tchoukalova YD, Ye J Diabetes 2012 61:137-44