Technical Data
C2390-25A
CD38 (Acute Lymphoblastic Leukemia Cells Antigen, ADP Ribosyl Cyclase, CADPr Hydrolase 1, Cyclic ADP Ribose Hydrolase, Ecto Nicotinamide Adenine Dinucleotide Glycohydrolase, I19 (Mouse), Lymphocyte Differentiation Antigen)
Description:
Murine CD38, a type II transmembrane glycoprotein, is a bifunctional ectoenzyme capable of catabolizing nicotinamide adenine dinucleotide (NAD+) to cyclic ADP-ribose (cADPR), and then hydrolyzing cADPR to adenosine diphosphoribose (ADPR).1-3 It is expressed at high levels on the surface of peripheral B-lineage cells, and at low density on germinal center B cells from unimmunized mice.4 It has also been reported to be expressed at moderate levels on NK cells, a proportion of peripheral T cells, and a subpopulation of thymocytes which are mostly TCRalpha/beta+, CD4-, CD8-.5, 6 Murine CD38 is also expressed by all Mac-1+ macrophages in the peritoneal cavities of unimmunized mice, but not by unstimulated bone-marrow-derived macrophages.4, 7 Monoclonal antibodies to CD38 have been shown to induce B- and T-cell proliferation, protect B cells from apoptosis, and inhibit B lymphopoiesis.2 ,5, 8

Applications:
Suitable for use in Flow Cytometry, Immunoprecipitation, Immunohistochemistry and Induction of B lymphocyte proliferation. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k5K253Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceMouseRat
Concentration:
~0.5mg/ml
Immunogen:
Purity:
Purified
Form
Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Specificity:
Recognizes mouse CD38 at 42kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Harada, N., L. Santos-Argumedo, R. Chang, J. C. Grimaldi, F. E. Lund, C. I. Brannan, N. G. Copeland, N. A. Jenkins, A. W. Heath, and R. M. Parkhouse. 1993. J. Immunol. 151:3111. 2. Santos-Argumedo, L., C. Teixeira, G. Preece, P. A. Kirkham, and R. M. E. Parkhouse. 1993. J. Immunol. 151:3119. 3. Howard, M., J. C. Grimaldi, J. F. Bazan, F. E. Lund, L. Santos-Argumedo, R. M. E. Parkhouse, T. F. Walseth, and H. C. Lee. 1993. Science 262:1056. 4. Lund, F., N. Solvason, J. C. Grimaldi, R. M. Parkhouse, and M. Howard. 1995. Immunol. Today 16:469. 5. Kirkham, P. A., L. Santos-Argumedo, M. M. Harnett, and R. M. Parkhouse. 1994. Immunology 83:513. 6. Bean, A. G. D., D. I. Godfrey, W. G. Ferlin, L. Santos-Argumedo, R. M. E. Parkhouse, M. C. Howard, and A. Zlotnik. 1996. Int. Immunol. 7:213. 7. Oliver, A. and J. Kearney. 1996. Personal Communication. 8. Kumagai, M., E. Coustan-Smith, D. J. Murray, O. Silvennoinen, K. G. Murti, W. E. Evans, F. Malavasi, and D. Campana. 1995. J. Exp. Med. 181:1101.