Technical Data
CD40 Ligand, soluble, Recombinant, Human (CD40 Ligand, CD40-L, CD40L, CD40LG, hCD40L, sCD40L, CD178, gp39, HIGM1, IGM, IMD3, T-BAM, T-cell Antigen GP39, TNF-related Activation Protein, TRAP, Tumor Necrosis Factor Ligand Superfamily Member 5, TNF Ligand Su
Molecular Biology Storage: -20°CShipping: Blue Ice
Recombinant human soluble CD40 ligand is a 16.3kD protein containing 149aa residues comprising the receptor binding TNF-like domain of CD40L.

CD40 is a member of the TNF receptor family. it is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells, and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40-Ligand is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to ?immunodeficiency with hyper-IgM, a disease characterized by failure to produce IgG, IgA and IgE. The human CD40L gene codes for a 261aa type II transmembrane protein, which contains a 22aa cytoplasmic domain, a 24aa transmembrane domain and a 215aa extracellular domain. The soluble form of CD40L is an 18kD protein comprising the entire TNF homologous region of CD40L. It is generated in vivo by an intracellular proteolytic processing of the full length CD40L.

CD40 Ligand (CD40L/TRAP, CD154) is a type II membrane protein. It effectuates the helper function of T cells on resting B cells. Recombinant human soluble CD40 Ligand (sCD40L/TRAP) is a 16.3kD protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L.

The CD40 antigen contains four homologous repeating cysteine-rich extracellular domains. It is a single chain glycoprotein. It is known to be a member of the tumor necrosis factor/nerve growth factor superfamily. It shows a significant homology to the Hodgkin’s disease-associated antigen, CD30. CD40 is present on all B cells except plasma cells. It is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 is found on normal and neoplastic B cells, strongly expressed by interdigitating cells (IDC), basal epithelial cells, carcinomas, macrophages, some endothelial cells, and follicular dendritic cells. It is a pan-B marker, absent only from terminally differentiated plasma cells.

CD40 receptor is expressed on antigen-presenting cells such as B cells, macrophages, dendritic cells and thymic epithelial cells. CD40 mediates stimulation of cell growth, differentiation and death. It also plays a role in regulating transcription in B cells.

CD40 is predicted to function in the process of B cell selection in the germinal center. Studies demonstrated that it induces strong homotypic adhesions in resting B cells. Together with Interleukin-4 (IL-4) maintains the cell cycle of B blasts and promotes the switch to IgE secretion. Puri ed B cells cultured in the CD40 system in the presence of IL-10 differentiate into plasma cells and secrete large amounts of immunoglobulins. The ligand for CD40 is a membrane glycoprotein on activated T cells that induces B cell proliferation and immunoglobulin secretion.

Biological Activity:
Assay 1: Determined by the stimulation of IL-12 induction by human PBMCs.
Assay 2: Determined by the does-dependent stimulation of IL-8 production by human PBMC. The expected ED50 for this effect is 5-10ng/ml.
Note: Results may vary with PBMC donor.

Amino Acid Sequence:

Storage and Stability:
Lyophilized powder may be stored at -20°C. Reconstitute with sterile 5-10mM sodium phosphate, 0.1% BSA or HSA. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Country of Origin:

Molecular Weight:
Source: Human, recombinant (E. coli)
Purity: ~98% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ug (1EU/ug)
Form: Supplied as a lyophilized powder from 10mM sodium phosphate, pH 7.5. Reconstitute with 5-10mM sodium phosphate, pH 7.5, to a concentration of 0.1-1mg/ml. Do not vortex. Further dilute with PBS, 0.1% BSA or HSA.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Ng-Cashin, J., et al., J. Immunol. 170(8): 4201-4208 (2003). 2. Andreakos, E., et al., Blood 101(3): 983-991 (2003). 3. Grant, E., et al., J. Exp. Med. 196(11): 1461-1471 (2002). 4. Skibinski, G., et al., Immunol. 102: 506-514 (2001).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.