Technical Data
CD40 Ligand, soluble, Recombinant, Human (CD40 Ligand, CD40-L, CD40L, CD40LG, hCD40L, sCD40L, CD178, gp39, HIGM1, IGM, IMD3, T-BAM, T-cell Antigen GP39, TNF-related Activation Protein, TRAP, Tumor Necrosis Factor Ligand Superfamily Member 5, TNF Ligand Su
Molecular Biology Storage: -20CShipping: RT
CD40L or CD154 is a membrane glycoprotein and differentiation antigen expressed on the surface of T-cells. The CD40 ligand stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also costimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2.
Recombinant Human CD40L is a non-glycosylated, Polypeptide chain containing 149 amino acids and having a molecular mass of 16.3D.

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Lys-Gly-Asp.

Biological Activity:
CD40L is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of IL-12 induction and IL-8 production by human PBMC (Peripheral Mononuclear) cells was found to be 5-10ng/ml.

Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm.
2. Analysis by RP-HPLC, using a standard solution of soluble CD40 as a Reference Standard.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: 97% by RP-HPLC, SDS-PAGE. Chromatographically purified.
Form: Supplied as a lyophilized powder from PBS, pH 7.0. Reconstitute with sterile ddH2O to 0.1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Prasad, M.L., et al., J. Clin. Pathol. 58: 90-92 (2005). 2. Toubi, E. & Shoenfeld, Y., Autoimmunity 37: 457-464 (2004). 3. Howard, L.M. & Miller, S.D., Autoimmunity 37: 411-418 (2004). 4. Hua, N., et al., Transplantation 78: 1463-1470 (2004). 5. Markees, T.G., et al., Transplantation 78: 1601-1608 (2004). 6. Diaz-Alderete, A., et al., J. Autoimmun. 23: 379-383 (2004).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.