Technical Data
C2394-03A
CD41 (IIB-IIIA)
Description:
The antibody precipitates a complex of molecular weights 122kD and 112kD when reduced and 87kD and 140kD when not reduced (1). The IIb-IIIa complex is a member of the integrin superfamily of adhesive receptors and mediates platelet-platelet aggregation. This complex recognizes the Arg-Gly-Asp (RGD) tripeptide sequence in adhesive glycoproteins such as fibronectin, fibrinogen and von Willebrand Factor thereby facilitating linkage between adjacent platelets. This antibody is useful in biochemical studies of the Glycoprotein IIb-IIIa complex and the enumeration of platelets. The antibody specifically inhibits ADP and collagen induced aggregation and release of ATP, and ADP induced platelet fibrinogen binding1. The antibody may also be used for immunoperoxidase staining of formalin fixed cell suspensions and frozen tissue sections.

Applications:
Suitable for use in Flow Cytometry, Immunofluorescence Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:25 Dilute with isotonic buffer. Use 50ul per 1x10e6 platelets in 100ul of buffer.
Indirect Immunofluorescence: 1:25 Dilute with isotonic buffer. Use 50ul per 1x10e6 platelets in 100ul of buffer.
Indirect Immunoperoxidase: 1:25
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG12Q948Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Human Platelet membranes
Purity:
Purified >95% by SDS-PAGE
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, 0.2% BSA.
Specificity:
Recognizes the human Glycoprotein IIb-IIIa complex on the surface of platelets and megakaryocytes. It is not reactive with T and B cells or with other haemopoietic cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Thurlow P.J. etal (1983): Detection of glycoprotein IIb and IIIa by monoclonal antibodies. Br. J. Haematol. 55:123134