Technical Data
C2395-20B
CD42b alpha (Glycoprotein Ib alpha, GPIba)
Description:
GPIb-alpha (platelet glycoprotein Ib-alpha; also CD42b and glycocalicin) is a platelet-derived 145kD member of the leucine-rich glycoprotein family of molecules. Mature human GPIb-alpha is a type I transmembrane protein 610 amino acids (aa) in length. It contains a 489 aa extracellular domain (ECD) (aa 17-505) and a 100 aa cytoplasmic region (aa 527-626). As part of a membrane complex, the GPIb-alpha ECD binds to von Willebrand Factor to impede platelet flow and promote clotting. The human GPIb-alpha ECD is approximately 50% aa identical to dog and mouse GPIb-alpha ECD.

Applications:
Suitable for use in Flow Cytometry, Direct ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 25ug/ml use 10ul to label 10e6 cells.
Direct ELISA: 0.5-1.0ug/ml detection limit for rhGPIb-alpha is ~1ng/well.
Western Blot: 1-2ug/ml detection limit for rhGPIb-alpha is ~ 5ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG18H211Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
NS0-derived rhGPIb-alpha. (aa 17 - 505)
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS.
Specificity:
Recognizes human GPIb-alpha.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.