Technical Data
C2398-01K
CD44 (CD 44, CDw44, ECM III, ECMRIII, Epican, Extracellular Matrix Receptor III, GP90 Lymphocyte Homing Adhesion Receptor, HCAM, HCELL, Heparan Sulfate Proteoglycan, Hermes Antigen, HUTCH1, Hyaluronate Receptor, Indian Blood Group, Inlu Related p80 Glycop
Description:
CD44 is a 90kD type I transmembrane protein, otherwise known as HCAM or CD44s. Various isoforms of CD44 exist. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul
Immunohistochemistry (Frozen): 1:100
Immunohistochemistry (Paraffin): 1:100; Requires antigen retrieval using heat treatment with sodium citrate buffer, pH 6 prior to staining of paraffin sections.
Western Blot: Detects a band at ~80kD in HeLa cell lysate.
Optimal dilutions to be determined by the researcher.

Recommended Negative Control:
I1904-83P1: IgG2a Murine Negative Control

Immunohistochemistry Positive Control:
Human Tonstil

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a10B1582Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Stimulated human leukocytes.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity:
Recognizes human CD44 at ~90kD expressed on leucocytes, erythrocytes, white matter of the brain and some epithelial cells of the breast and small intestine. Reacts with epitope 3, a protease resistant epitope on CD44. Species Crossreactivity: baboon, African green monkey
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Denning, S.M. et al. (1995) AS10 CD44 and CD45R Cluster report. In Leucocyte Typing V. White cell differentiation antigens. Eds Schlossman, S.F. et al. Oxford University Press. Volume 2:1713-1719.