Technical Data
C2398-85
CD44S (HCAM, Homing Cell Adhesion Molecule, Pgp-1, Phagocytic Glycoprotein-1, Hermes Antigen, Lymphocyte Homing Receptor, ECM-III, HUTCH-1)
Description:
CD44 (H-CAM, Homing Cell Adhesion Molecule) is a 85-90kD transmembrane protein. The human CD44 gene contains 19 exons; 1-5 and 15-19 are expressed by wild-type CD44, also known as the standard hemopoietic form (CD44s).

Applications:
Suitable for use in ELISA, RIA, Flow Cytometry, Immunoflourescence, Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
ELISA: 1:2000
RIA: 1:5000
Flow Cytometry: 1:1000
Immunoflourescence: 1:200
Immunohistochemistry (Paraffin): 1:250 Antigen retrieval is usually not needed.
Immunoprecipitation: 1:200
Western Blot: 1:1000. Non-reduced only. ~90kD band detected; Blocking PBS/5% milk protein. Overnight incubation.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
TypeIsotypeCloneGrade
MabIgG2b2Q1374Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRat
Concentration:
~1mg/ml
Immunogen:
Purified human blood lymphocyte CD44
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.5, 0.01% sodium azide.
Specificity:
Recognizes human CD44 (85-95kD) gp90 Hermes and mouse gp85 (Pgp-1). Reacts with the standard CD44 isoform that lacks exon sequences v1-v10. Species Crossreactivity: rabbit, porcine, mouse, rat, canine, bovine cells/tissue
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
11.Bourguignon, L.Y.W., Lokeshwar, V.B., Chen, X. and Kerrick, W.G.L., J. Immunology, (1993) 151:6634–6644.