Technical Data
C2398-86
CD44V10 (HCAM, Homing Cell Adhesion Molecule, Pgp-1, Phagocytic Glycoprotein-1, Hermes Antigen, Lymphocyte Homing Receptor, ECM-III, HUTCH-1)
Description:
Recent studies indicate that the expression of CD44 on the cell surface changes profoundly during tumor metastasis, particularly during the progression of various carcinomas. Sequencing analysis reveals that CD44 exists in different forms (at least 15 isoforms have been identified) that are variants of the common or standard hemopoietic form CD44s. Specifically, the human CD44 gene contains 19 exons, out of which 10 exons can potentially be alternatively spliced. In particular, CD44s contains exons 115 and exons 1519. The other exons (e.g. 614) are also spliced in a variety of different cells. For example, epithelial cells contain exons 1214 inserted into the CD44s transcripts. This isoform has been designated as CD44E. Other CD44 variants CD44v) have the same sequences at the two ends of the molecule, but differ in a middle region (with different numbers of additional exons) located on the external side of the cell membrane. It has been suggested that expression of CD44 variants with the additional exon 14 (so-called v10) is responsible for tumor progression and metastasis. In fact, CD44v10 expression has been used as an indicator of metastasis.

Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, RIA and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 1:200
Flow Cytometry: 1:1000
ELISA: 1:2000
Western Blot: 1:10,000
Immunohistochemistry (Paraffin): 1:250
Immunoprecipitation: 1:200
RIA: 1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to human CD44v10.
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.01% sodium azide.
Specificity:
Recognizes human CD44v10 (~116kD). Species Crossreactivity: rabbit, porcine, mouse, rat, canine and bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. J. Biol. Chem. (1996) 271:23853-23864. 2. Dall, P, et al. (1994). Cancer Research (1994) 54:3337-3341. 3. Screaton, G R, et al. (1992) Proc. Natl. Acad. Sci. (USA) 89:12160-12164. 4. Arch, R, et al. (1992) Science 257:682-685