Technical Data
CD44V3 (HCAM, Homing Cell Adhesion Molecule, Pgp-1, Phagocytic Glycoprotein-1, Hermes Antigen, Lymphocyte Homing Receptor, ECM-III, HUTCH-1)
CD44 variant 3 is a cell surface glycoprotein expressed on lymphocytes, monocytes and granulocytes. The CD44 molecule belongs to a family of cellular adhesion molecule found on a wide range of normal and malignant cells in epithelial, mesothelial and hematopoietic tissues. CD44 is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA. The expression of specific cell adhesion molecule CD44 splice variants has been shown to be associated with metastasis and poor prognosis in certain human malignancies, such as breast cancer.

Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:200
Flow Cytometry: 1:1000
ELISA: 1:2000
Western Blot: 1:10,000
Immunoprecipitation: 1:200
Immunohistochemistry (Paraffin-embedded): 1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to human CD44v3.
Supplied as a liquid in PBS, pH 7.5, 0.01% sodium azide.
Recognizes human CD44v3 (~200kD). Species Crossreactivity: rabbit, porcine, mouse, rat, canine and bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Cancer Research 54: 3337-3341 (1994). 2. Proc. Natl. Acad. Sci. USA 89: 12,16012,164 (1992). 3. Science 257: 682685 (1992).