Technical Data
C2402-02
CD47
Description:
CD47 antigen, also known as integrin associated-protein (IAP), is expressed on all hematopoietic cells, including leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. CD47 may play a role as a signal transducer in the regulation of cation fluxes across cell membranes and in the chemotactic and adhesive interactions of leukocytes with endothelial cells.

MW of Antigen: 47-52kD (reduced) and 45-50.5, 110kD (non-reduced)

Epitope: Ig domain

Applications and Recommended Dilutions:
Blocks binding of SIRPa4,5
Inhibits in assays in which CD47-integrin association is required6,7,8
Flow Cytometry
Immunofluorescence (Methanol fixed only)
Immunoprecipitation (Native only, Use Protein G) (Ab 2ug/mg protein lysate) The optimal dilution for a specific application should be determined by the investigator.

Positive Control: Tonsil

Cellular Localization: Cell membrane

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20C or colder. Aliquots are stable for at least 12 months at-20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k3F95Affinity Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Intact CD47 purified from placenta.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C2402-02X
Specificity:
Blocks binding of SIRP alpha and in assays of integrin association. Species Crossreactivity: human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Brown, E. et al. 1990 J. Cell Biol. 111:2785. 2. Kishimoto, T. et al 1998. Leucocyte Typing VI: Garland Press, London. 3. Schlossman, S. et al. 1995. Leucocyte Typing V: Oxford University Press, New York. 4. Yoshida, et al. (2002) J Immunol, 168::321320. 5. Latour et al. (2001) J Immunol, 167:254754. 6. J. Cell Biol, 135: 533544, (1996). 7. J. Cell Biol, 134: 13131322, (1996). 8. J Immunol, 163:60786085, (1999).