Technical Data
C2402-03F
CD47
Description:
Applications:
Suitable for use in Flow cytometry, Western blot (non-reducing) and Immunohistochemistry (). Other applications have not been tested.

Recommended Dilutions:
Western Blot: Non-reducing
Immunohistochemistry: frozen sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgM8J217Highly Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
COS-7 (African green monkey) cells
Purity:
Purified by CHT chromatography and precipitation methods.>95% pure (SDS-PAGE).
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide
Specificity:
Recognizes human CD47 (Integrin Associated Protein), a 50-55kD membrane adhesion molecule (thrombospondin receptor; immunoglobulin supergene family) expressed on leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. Reacts with pig and non-human primates.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Leukocyte Typing VII, (2002), Mason D. et al., (Eds.), Oxford University Press
2. Leksa, V., et al., (2002), “The N terminus of mannose 6-phosphate/insulin-like growth factor 2 receptor in regulation of fibrinolysis and cell migration”, J. Biol. Chem., 277(43): 40575-40582