Suitable for use in Flow cytometry, Western blot (non-reducing) and Immunohistochemistry (). Other applications have not been tested.
Western Blot: Non-reducing
Immunohistochemistry: frozen sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
| Not determined|
|COS-7 (African green monkey) cells|
|Purified by CHT chromatography and precipitation methods.>95% pure (SDS-PAGE). |
|Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide|
|Recognizes human CD47 (Integrin Associated Protein), a 50-55kD membrane adhesion molecule (thrombospondin receptor; immunoglobulin supergene family) expressed on leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. Reacts with pig and non-human primates.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Leukocyte Typing VII, (2002), Mason D. et al., (Eds.), Oxford University Press|
2. Leksa, V., et al., (2002), “The N terminus of mannose 6-phosphate/insulin-like growth factor 2 receptor in regulation of fibrinolysis and cell migration”, J. Biol. Chem., 277(43): 40575-40582