Technical Data
C2402-03G2
CD47 (CD47 Antigen, Antigenic Surface Determinant Protein OA3, Integrin Associated Protein, IAP, Integrin Associated Signal Transducer, Leukocyte Surface Antigen CD47, MER6, OA3, Protein MER6, Rh-related Antigen)
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Technical Data |
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C2402-03G2 |
CD47 (CD47 Antigen, Antigenic Surface Determinant Protein OA3, Integrin Associated Protein, IAP, Integrin Associated Signal Transducer, Leukocyte Surface Antigen CD47, MER6, OA3, Protein MER6, Rh-related Antigen) |
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Description: CD47, also known as integrin-associated protein, is a 50kD glycoprotein expressed on all hematopoietic cell lines, epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. CD47 is involved in several cellular processes including regulation of T cell and dendritic cell activation, regulation of neutrophil (PMN) chemotaxis, caspase-independent apoptosis, and may act as a signal transducer in the regulation of cation fluxes across cell membranes. CD47 has been identified as a ligand for the inhibitory macrophage receptor known as signal regulatory protein alpha (SIRPalpha) and as a receptor for thrombospondin-1. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry (frozen), and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:25-1:100; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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