Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested.
Flow Cytometry: 1:10-1:100 Use 10ul to label 10e6 cells in 100ul.
Optimal dilution determined by the researcher.
Spleen cells from immunized Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Recommended Secondary Reagents:
IgG Goat anti mouse IgG:FITC (rat adsorbed)
IgG Goat anti mouse IgG:RPE (rat adsorbed)
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|100ug||4°C (-20°C Glycerol)||Blue Ice||Rat||Mouse|
|Purified by Protein G affinity chromatography.|
|Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.|
|Recognizes the rat homologue of human CD47, which has been identified as the ligand for the rat signal regulatory protein (SIRP). Rat CD47 is expressed primarily by lymphocytes and macrophages but has also been detected at high levels on brain endothelial cells.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Vernon-Wilson, E. F. et al. (2000). CD47 is a ligand for rat macrophage membrane signal regulatory protein SIRP (OX41) and human SIRP alph a 1. Eur. J. Immunol. 30: 2130-2137. 2. de Vries, H.E. et al. (2002). Signal regulatory protein alpha - CD47 interactions are required for the transmigration of monocytes across cerebral endothelium. J. Immunol. 168: 5832 - 5839.|