Fusion Partners: Spleen cells from immunized AO Rats immunized were fused with cells from the Rat Y3 myeloma cell line.
Recommended Negative Controls: Rat IgG2a Negative Control: RPE
Flow Cytometry: Neat
Optimal working dilutions to be determined by researcher.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
| As reported|
|Recombinant soluble mouse CD48.|
|Purified by Protein G affinity chromatography.|
|Supplied as a lyophilized powder from PBS, pH 7.2, 0.09% sodium azide, 1% BSA. Labeled with R. Phycoerythrin (RPE). Reconstitute with 1ml sterile 40-50% glycerol, ddH2O.|
|MRC OX-78 labels mouse thymocytes, B and T cells and macrophages. CD48 is a ligand for the mouse CD2 cell surface antigen. |
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Kato et al. (1992). J. Exp. Med. 176: 1241-1249.|