Technical Data
C2404-06B
CD49b (Integrin alpha2, ITGA2, alpha-2 subunit of VLA-2 Receptor, Platelet Antigen B)
Description:
Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing alpha integrin 2 combines with beta integrin 1 to form a collagen-binding integrin referred to as glycoprotein Ia/IIa (gpIa/IIa) when expressed on platelets, and very late (activation) antigen 2 ('VLA-2') when found on T-cells. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. CD49b is expressed approximately 50% of monocytes and most adherent cell lines.
CD49b forms a non-covalent association with the 130kD VLA beta chain (CD29) in the VLA-2 complex. The CD49b/CD29 complex is also known as a2/b1 and VLA-2. The complex mediates binding of cells to collagen (types I, II, III, and IV) and to laminin. The complex of CD49b associated with CD29 (integrin b1) is a receptor for laminin and collagen. This complex is expressed by some CD4-positive T-cells, natural killer cells, epithelial cells and platelets.

Applications:
Suitable for use in Flow Cytometry, ELISA, Immunoprecipitation, Immunofluorescence, Immunohistochemistry and Blocking. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 0.5ug/10e6 cells in 100ul
Immunohistochemistry: Frozen tissues
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2Q1014Affinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceMouseHamster
Concentration:
~0.5mg/ml
Immunogen:
Mouse colon carcinoma cell line Colon26.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes the 150kD mouse CD49b (Integrin a2).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Noto, K. .et al., Int. Immunol. 7:835-842 (1995). 2. Miyake S. et al., Eur. J. Immunol. 24:2000-2005(1994).