Technical Data
CD49b (Integrin alpha2, ITGA2, alpha-2 subunit of VLA-2 Receptor, Platelet Antigen B)
Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing alpha integrin 2 combines with beta integrin 1 to form a collagen-binding integrin referred to as glycoprotein Ia/IIa (gpIa/IIa) when expressed on platelets, and very late (activation) antigen 2 ('VLA-2') when found on T-cells. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. CD49b is expressed approximately 50% of monocytes and most adherent cell lines.

CD49b forms a non-covalent association with the 130kD VLA beta chain (CD29) in the VLA-2 complex. The CD49b/CD29 complex is also known as a2/b1 and VLA-2. The complex mediates binding of cells to collagen (types I, II, III, and IV) and to laminin. The complex of CD49b associated with CD29 (integrin b1) is a receptor for laminin and collagen. This complex is expressed by some CD4-positive T-cells, natural killer cells, epithelial cells and platelets.

Applications: Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Attachment inhibition assays: Fibroblasts, epithelial cells, endothelial cells, and non-activated platelets on collagen types I, III, IV, VI and laminin.

Immunohistochemistry (immunofluorescence): Fresh frozen or paraffin embedded tissue (protease digestion is necessary when using paraffin). For extensive dilution, protein-containing or other stabilizing medium should be used.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG12Q1009Affinity Purified
100ug-20CBlue IceHumanMouse
Purified from ascites fluid by protein A chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Recognizes human a2 integrin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Wayner, E. A., et al. (1988). J. Cell Biol., 107:1881. 2. Sanchez-Mateos, P., et al. (1993). J. Immunol., 151:3817. 3. Sriramarao, P., et al. (1993). J. Biol. Chem., 268:22036. 4. Kapron-Bras, C., et al. (1993). J. Biol. Chem., 368:20701