Technical Data
CD49b (Integrin alpha2, ITGA2, alpha-2 subunit of VLA-2 Receptor, Platelet Antigen B)
Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing alpha integrin 2 combines with beta integrin 1 to form a collagen-binding integrin referred to as glycoprotein Ia/IIa (gpIa/IIa) when expressed on platelets, and very late (activation) antigen 2 ('VLA-2') when found on T-cells. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. CD49b is expressed approximately 50% of monocytes and most adherent cell lines.

CD49b forms a non-covalent association with the 130kD VLA beta chain (CD29) in the VLA-2 complex. The CD49b/CD29 complex is also known as a2/b1 and VLA-2. The complex mediates binding of cells to collagen (types I, II, III, and IV) and to laminin. The complex of CD49b associated with CD29 (integrin b1) is a receptor for laminin and collagen. This complex is expressed by some CD4-positive T-cells, natural killer cells, epithelial cells and platelets.

Applications: Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Attachment inhibition assays: Fibroblasts, epithelial cells, endothelial cells, and non-activated platelets on collagen types I, III, IV, VI and laminin.

Immunohistochemistry (immunofluorescence): Fresh frozen or paraffin embedded tissue (protease digestion is necessary when using paraffin). For extensive dilution, protein-containing or other stabilizing medium should be used.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG12Q1009Affinity Purified
100ug-20CBlue IceHumanMouse
Purified from ascites fluid by protein A chromatography
Supplied as a liquid in 0.01M PBS. No preservative added.
Recognizes human a2 integrin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wayner, E. A., et al. (1988). J. Cell Biol., 107:1881. 2. Sanchez-Mateos, P., et al. (1993). J. Immunol., 151:3817. 3. Sriramarao, P., et al. (1993). J. Biol. Chem., 268:22036. 4. Kapron-Bras, C., et al. (1993). J. Biol. Chem., 368:20701