Technical Data
C2404-07B
CD49b (Integrin alpha2, ITGA2, alpha-2 subunit of VLA-2 Receptor, Platelet Antigen B)
Description:
Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing alpha integrin 2 combines with beta integrin 1 to form a collagen-binding integrin referred to as glycoprotein Ia/IIa (gpIa/IIa) when expressed on platelets, and very late (activation) antigen 2 ('VLA-2') when found on T-cells. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. CD49b is expressed approximately 50% of monocytes and most adherent cell lines.

CD49b forms a non-covalent association with the 130kD VLA beta chain (CD29) in the VLA-2 complex. The CD49b/CD29 complex is also known as a2/b1 and VLA-2. The complex mediates binding of cells to collagen (types I, II, III, and IV) and to laminin. The complex of CD49b associated with CD29 (integrin b1) is a receptor for laminin and collagen. This complex is expressed by some CD4-positive T-cells, natural killer cells, epithelial cells and platelets.

Applications:
Suitable for use in Indirect Immunofluorescence, FACS, Immunohistochemistry and ELISA. Other applications have not been tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Molecular Weight: 130,170kD

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG12Q1008Highly Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Human T lymphocytes
Purity:
Purified by ammonium sulfate precipitation and ion exchange chromatography.
Form
Supplied as a liquid in 20mM Tris, 150mM sodium chloride, pH 8.0, 10mg/ml BSA, 0.1% sodium azide.
Specificity:
Directed against the a2 human integrin (CD49b antigen, GPIa or VLA-2 alpha chain). Reacts with platelets, long-term cultivated T lymphocytes and activated T lymphocytes. Reacts with thymocytes, epithelial cells of a variety of tissues, peripheral nerves, fibroblasts, osteoclasts, glomerular mesangium and most non-hematopoietic adherent cell lines.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. J. Biol. Chem. 263:4516 (1988). 2. Blood 73:1235 (1989). 3. J. Cell Biol. 108:1917 (1989).