Technical Data
C2404-55
CD49d (Integrin alpha4, VLA4, LHR, Lymphocyte Homing Receptor)
Description:
CD49d (VLA-4) inhibits adhesion of rat lymphocytes to cytokine-activated endothelial cells. It blocks binding of rat lymphocytes to purified VCAM-1 and to the CS-1 peptide of fibronectin. VLA-4 is a leukocyte integrin which plays an important role in lymphocyte adhesion and activation.

Applications:
Suitable for use in ELISA. Other applications have not been tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Hybridoma:
Hybridization of P3U1 mouse myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG18.S.168Highly Purified
SizeStorageShippingSourceHost
200ug4°C (-20°C Glycerol)Blue IceRatMouse
Concentration:
1mg/ml
Immunogen:
Rat peritoneal lymphocytes
Purity:
>95%. Purified from ascites by column chromatography.
Form
Supplied as a liquid in PBS.
Specificity:
Recognizes rat VLA4 (very late antigen-4) alpha 4 chain (CD49d: 150kD) which is assoclated with beta 2 (CD29; 130kD).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Issekutz, T. & Wykretowlcz, A, J. Immunol., 147:109 (1991)
2. Issekutz, T., J. Immunol., 147:4178 (1991)
3. Paul, L.C. et al., Transplantation Proceedings, 25:1, 813 (1993)
4. Paul, L.C. et al., Transplantation Proceedings, 55:1196 (1993)