Technical Data
C2405-01V
CD49e (Integrin alpha5) (FITC)
Description:
CD49e is the integrin alpha5 chain, a 135/25kD heterodimer, that associates noncovalently with CD29, the integrin beta1 subunit, to form the alpha5beta1 very late antigen-5 (VLA-5) complex, a very well established fibronectin receptor that is expressed on many cell types, including thymocytes, T cells, monocytes and platelets. Expression of the VLA-5 heterodimer is increased on activated and memory T cells. It is also found on very early B cells and on activated B lymphocytes. CD49e/CD2-mediated binding to fibronectin provides a costimulatory signal to T cells. It also enhances Fc-gammaR- and complement receptor-mediated phagocytosis, and is involved in monocyte migration into extracellular tissues.1-6

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Function blocking. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 20ul/10e6 cells
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG2b5K288Purified
SizeStorageShippingSourceHost
100Tests-20CBlue IceHumanMouse
Concentration:
~100tests/ml
Immunogen:
Human CD49e.
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.1% sodium azide and a stabilizing agent. Labeled with FITC.
Specificity:
Recognizes human CD49e.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Schlossman, S., L. Bloumsell, W. Gilks, J.M. Harlan, C. Kishimoto, J. Ritz, S. Shaw, R. Silverstein, T. Springer, T.F. Tedder, and R.F. Todd, eds. 1995. Leukocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, Oxford. 2. Hemler, M.E. 1990. Annu. Rev. Immunol. 114:365 3. 155. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD49e Section, Academic Press, New York, p. 265. 4. te Velde, A.A., J.P. Klomp, B.A. Yard, J.E. de Vries, and C.G. Figdor. 1988. J. Immunol. 140:1548. 5. Nermut, M.V., N.M. Green, P. Easton, S.S. Yamada, and K.M. Yamada. 1988. EMBO J. 7:4093. 6. Hynes, R.O. 1992. Cell 69:11.