Technical Data
C2405-02D
CD49e (Integrin alpha5)
Description:
CD49e is a type I membrane protein that associates non-covalently with Integrin beta 1 (CD29) to form the heterodimer CD49e/CD29 (VLA-5). VLA-5 is expressed on mast cells, splenic B cells, thymocytes, T-cells and monocytes. The VLA-5 heterodimer forms the fibronectin receptor, which plays an important role in cell adhesion and migration.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:500-1:2000
Western Blot: 1:1000 detects integrin alpha 5 on 10ug of HUVEC lysates. Use denatured but non-reduced samples to detect full length product. ECL; BSA blocking suggested.
Immunoprecipitation: Use 5ul antibody for 5x10e6 cells.
Immunohistochemistry (Acetone fixed tissues): 1:1000
Immunocytochemistry: 1:1000
Optimal dilutions to be determined by the researcher.

Control:
Mouse 3T3 fibroblasts, skin (basement membrane)

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Synthetic peptide derived from the C-terminal sequence (cytoplasmic domain) of the human alpha 5 Integrin subunit (NP_002196).
Purity:
Serum
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.05% sodium azide.
Specificity:
Recognizes human Integrin alpha 5 at ~114kD. No crossreactivity to alpha 1, alpha 2, alpha 3, alpha 4, alpha 6 or alpha V. The antibody recognizes an epitope on the cytoplasmic domain of the protein, and is therefore, intracellular. Species Crossreactivity: chicken, mouse, rat and hamster.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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