Technical Data
CD49e (Integrin alpha5)
CD49e is a type I membrane protein that associates non-covalently with Integrin beta 1 (CD29) to form the heterodimer CD49e/CD29 (VLA-5). VLA-5 is expressed on mast cells, splenic B cells, thymocytes, T-cells and monocytes. The VLA-5 heterodimer forms the fibronectin receptor, which plays an important role in cell adhesion and migration.

Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Immunofluorescence and RIA. Other applications not tested.

Recommended Dilutions:
ELISA: 1:500-1:2000
Western Blot: 1:1000 detects integrin alpha 5 on 10ug of HUVEC lysates. Use denatured but non-reduced samples to detect full length product. ECL; BSA blocking suggested.
Immunoprecipitation: Use 5ul antibody for 5x10e6 cells.
Immunohistochemistry (Acetone fixed tissues): 1:1000
Immunocytochemistry: 1:1000
Optimal dilutions to be determined by the researcher.

Mouse 3T3 fibroblasts, skin (basement membrane)

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20°CBlue IceHumanRabbit
Not Determined
Synthetic peptide derived from the C-terminal sequence (cytoplasmic domain) of the human alpha 5 Integrin subunit (NP_002196).
Supplied as a liquid in PBS, 0.1% BSA, 0.05% sodium azide.
Recognizes human Integrin alpha 5 at ~114kD. No crossreactivity to alpha 1, alpha 2, alpha 3, alpha 4, alpha 6 or alpha V. The antibody recognizes an epitope on the cytoplasmic domain of the protein, and is therefore, intracellular. Species Crossreactivity: chicken, mouse, rat and hamster.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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