Technical Data
C2405-03
CD49f (Laminin Receptor, Integrin alpha6, VLA alpha6, Platelet gpIc)
Description:
Integrins are a family of dimeric, transmembrane proteins that mediate cell-cell and extracellular matrix adhesion. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. The integrin family is composed of at least 15 alpha and 8 beta subunits that may form over twenty different alpha-beta non-covalently bound dimeric combinations on the cell surface. The alpha subunits all have some homology to each other, as do the beta subunits. Both of the subunits contribute to the binding of the ligand. Integrin alpha subunits contain seven weak sequence repeats in the N-terminal region, which may be important in ligand binding, and have been predicted to fold cooperatively into a single beta-propeller domain with seven beta-sheets. In normal tissue, Integrin alpha-6/beta-1 is a receptor for laminin on human platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome. Various disease states involving epithelial cells have been shown to be associated with alterations in alpha 6 integrin-containing heterodimers.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Inhibition. Other applications have not been tested.

Recommended Dilutions:
Flow Cytometry: 1:25-1:50; Use 10ul per 10e6 cells in 100ul.
Immunohistochemistry: Frozen tissue sections
Inhibition of laminin binding: 40ug/ml.
Optimal dilutions to be determined by researcher.

Positive Control:
Human tonsil (for Immunohistochemistry)

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

TypeIsotypeCloneGrade
MabIgG2a2Q957Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
BALB/c mouse mammary tumor cells.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity:
Recognizes human Cdw49f-antigen, 120kD, (GP Ic or VLA-6 alpha-chain). Inhibits platelet adhesion to laminin. Reacts with platelets, megakaryocytes, T lymphocytes and common Acute Lymphoblastic Leukemia cells (alpha-6 beta-1). Reacts with epithelial cells of a variety of tissues, peripheral nerves, microvascular endothelial cells, placenta cyto- and syncytotrophoblasts. Species Crosseactivity: Mouse, Cynomologus monkey, porcine, and canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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