Technical Data
C2407-51
CD52 (CAMPATH 1)
Description:
CD52 is a 25-29kD glycosylphosphatidylinositol (GPI)- anchored single chain, a remarkably small glycoprotein, which is typically expressed at high levels on thymocytes, lymphocytes, NK cells and monocytes/macrophages. It is expressed on some granulocytes, on epithelial cells of the male reproductive tract and on the majority of lymphoid malignancies, but not on erythrocytes and platelets. A somatic defect in the synthesis of GPI anchors (for example lymphocytes from patients with paroxysmal nocturnal haemoglobinuria (PNH)) may cause lack of expression of CD52 on the cell surface. CD52 may then be used as an index to diagnose PNH besides CD55 and CD59. All CD52 antibodies are characterized by a strong lytic activity with the human complement, irrespective of antibody isotype.

Applications:
Suitable for use in Indirect Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 0.125ug labels 100ul peripheral blood using Indirect Immunofluorescence.
Immunohistochemistry: Acetone-fixed frozen and formalin-fixed, paraffin sections.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b5F44Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
human tonsil
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.0, 0.1% sodium azide.
Specificity:
Recognizes human CD52. Reacts with normal human peripheral blood lymphocytes (90.6%). Does not react with normal human peripheral blood granulocytes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tadamitsu K, K. Hitoshi, A.E.G.Kr. van dem Borne, et al., eds., 1997. Leukocyte Typing VI: White Cell Differentiation Antigens. P 514516 1152 Garland Publishing, Inc., New York.