Technical Data
C2409-14B
CD54 (ICAM-1, Intercellular Adhesion Molecule 1, Human Rhinovirus Receptor, Precursor, BB2, Major Group Rhinovirus Receptor, P3.58)
Description:
CD54 permits antigen-independent adhesion between lymphocytes and their targets and may be used to study cell-cell interactionsor for rhinovirus and malarial studies. The LFA-1 binding site is located in domain 1 of ICAM-1. CD54 antigen is a membrane glycoprotein of ~90kD with a wide tissue distribution that includes vascular endothelium and many cells of the immune system. CD54 is weakly expressed on resting peripheral blood lymphocytes. Upon activation by mitogens, the CD54 antigen is strongly expressed on B-cells, T-cells, macrophages and granulocytes.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:50-1:100. Use 10ul to label 10e6 cells in 100ul.
Immunohistochemistry (Frozen): 1:50-1:100
Optimal working dilutions to be determined by researcher.

Recommended Positive Control:
Human Tonsil

Recommended Secondary Antibodies:
I1904-65W: IgG, F(ab)2 (HRP) Pab Rb xMo
F(ab')2 rabbit anti mouse IgG: RPE conjugate

Recommended Negative Control:
I1904-78R: IgG1 Murine Negative Control

Hybridoma:
Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13H1547Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human monocytes
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity:
Recognizes human CD54, a 90kD glycoprotein. Expressed by many cells following activation by inflammatory mediators. Blocks CD54 function. Species Crossreactivity: porcine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. J. Cell Biol. (1992) 116: 1527-1535. 2. Cell (1992) 68: 71-81. 3. Treadwell, M.D. et al. (1996). Increased neutrophil adherence to endothelial cells exposed to asbestos. Toxicol Appl. Pharmacol. 139: 62-70.