Technical Data
CD54 (Intercellular Adhesion Molecule, ICAM-1) (Biotin)
CD54, a member of the immunoglobulin superfamily of cell surface receptors and also known as Intercellular Adhesion Molecule-1 (ICAM-1), is a type I transmembrane glycoprotein that mediates important cell/cell interactions. CD54 is expressed on endothelial cells, dendritic cells, keratinocytes and lymphocytes. Its expression is upregulated by inflammatory mediators such as IFN-gamma, IL-1, TNF-alpha, lipopolysaccharide and phorbol esters. Endothelial CD54 contributes to the extravasation of leukocytes from blood vessels, particularly in areas of inflammation. CD54 on antigen-presenting cells contributes to antigen-specific T-cell activation, presumably by enhancing interactions between T cell and antigen-presenting cells. These adhesion reactions are mediated via the binding of CD54 to its major ligands, LFA-1 and Mac-1.

Suitable for use in Western Blot, Immunoprecipitation, Flow Cytometry, Immunohistochemistry, and in vitro blocking studies. Other applications have not been tested.

Recommended Dilution:
Flow Cytometry: 1ug/10e6 cells
Immunohistochemistry: Frozen sections.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
500ug-20CBlue IceMouseRat
Mouse CD54
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Labeled with Biotin.
Recognizes mouse CD54/ICAM-1, Mr 95kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Takei, F. 1985. J. Immunol. 134:1403. 2. Horley, K.J., C. Carpenito, B. Baker, F. Takei. 1989. EMBO. J. 8:2889. 3. Welder, Clayton A., D.H.S. Lee, F. Takei. 1993. J. Immunol. 150:2203. 4. Qin, Lan, et. al. 1996. J. Immunol. 157:5016.