Technical Data
C2409-19J
CD54 (Intercellular Adhesion Molecule, ICAM-1) (Biotin)
|
Technical Data |
|
C2409-19J |
CD54 (Intercellular Adhesion Molecule, ICAM-1) (Biotin) |
|
|
Description: CD54, a member of the immunoglobulin superfamily of cell surface receptors and also known as Intercellular Adhesion Molecule-1 (ICAM-1), is a type I transmembrane glycoprotein that mediates important cell/cell interactions. CD54 is expressed on endothelial cells, dendritic cells, keratinocytes and lymphocytes. Its expression is upregulated by inflammatory mediators such as IFN-gamma, IL-1, TNF-alpha, lipopolysaccharide and phorbol esters. Endothelial CD54 contributes to the extravasation of leukocytes from blood vessels, particularly in areas of inflammation. CD54 on antigen-presenting cells contributes to antigen-specific T-cell activation, presumably by enhancing interactions between T cell and antigen-presenting cells. These adhesion reactions are mediated via the binding of CD54 to its major ligands, LFA-1 and Mac-1. Applications: Suitable for use in Western Blot, Immunoprecipitation, Flow Cytometry, Immunohistochemistry, and in vitro blocking studies. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 1ug/10e6 cells Immunohistochemistry: Frozen sections. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
|
|||||||||||
|
||||||||||||
|
||||||||||||
|
||||||||||||