Technical Data
C2409-26
CD54 (Intercellular Adhesion Molecule, ICAM-1) (Biotin)
Description:
ICAM-1 permits antigen-independent adhesion between lymphocytes and their targets and may be used to study cell-cell interactionsor for rhinovirus and malarial studies. The LFA-1 binding site is located in domain 1 of ICAM-1. CD54 antigen is a membrane glycoprotein with a wide tissue distribution that includes vascular endothelium and many cells of the immune system. CD54 is weakly expressed on resting peripheral blood lymphocytes. Upon activation by mitogens, the CD54 antigen is strongly expressed on B-cells, T-cells, macrophages and granulocytes. ICAM-1 has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells and on a variety of other cell types including activated fibroblasts, EC, macrophages and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs. Inhibits adhesion of infected RBCs and LFA-1 to ICAM-1. CD54 is the receptor for rhinoviruses and malaria.

Applications:
Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Also suitable for use in studies of inflammation, lymphocyte differentiation and activation. Other applications have not been tested.

Recommended Dilution:
Flow Cytometry: 1ug to stain 1x10e6 cells.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a1.BB.705Affinity Purified
SizeStorageShippingSourceHost
500ug4°C (-20°C Glycerol)Blue IceMouseRat
Concentration:
~0.5mg/ml
Immunogen:
CD54 (ICAM-1)
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 1% BSA, 2mM EDTA, 0.1% sodium azide. Labeled with biotin.
Specificity:
Recognizes the Mr 95kD cell surface glycoprotein known as intracellular adhesion molecule ICAM-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kuhlman, P. et al. (1991) J. of Immunol. 146(6):1773 2. Schynius, A. et al. (1993) J. Immunol. 150:655 3. Harlan, J. M. and Liu, D. Y. (1992) Adhesion: Its Role in In ammatory Disease, W. H. Freeman and Co., New York, p. 154