Technical Data
C2410-02
CD55 (DAF, Decay Accelerating Factor)
Description:
CD55 is a cell surface antigen. It is a GPI linked molecule also known as Decay Accelerating Factor (DAF). CD55 is expressed by a wide range of cell types. Erythrocyte expression is very weak.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Flow Cytometry: 1:5-1:12.5. Use 20ul to label 10e6 cells in 100ul.
Immunohistochemistry (frozen): 1:50-1:500
Optimal dilutions to be determined by researcher.

Note: Use of this reagent to stain erythrocytes is not recommended.

Recommended Secondary Reagents:(FC)
I1904-66A: IgG, F(ab’)2, X-Adsorbed (FITC) Pab Rb xMo
I1903-13A: IgG, F(ab’)2, X-Adsorbed (PE) Pab Gt xMo

Recommended Secondary Reagents:(IHC)
I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab Rb xMo

Recommended Positive Control Tissue: (IHC)
Human Tonsil

Recommended Negative Controls: (IHC)
I1904-78R: IgG1 Negative Control Mab Mo x

Source: Tissue culture

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG13H1563Affinity Purified
SizeStorageShippingSourceHost
200ug4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
K562 cells
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes the human CD55 cell surface antigen.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lublin, D.M. and Atkinson, J.P. (1989). Ann. Rev. Immunol. 7: 35-38. 2. Daniels, G.L. (1989). Vox Sang. 56: 205-211. 3. Holmes, C.H., et al. (1990). J. Immunol. 144: 3099-3105.