Technical Data
CD56 (NCAM-1, Neural Cell Adhesion Molecule)
The neural cell adhesion molecule (NCAM/CD56) has been shown to mediate homophilic cell-cell adhesion in the neuroendocrine system with interactions among immunoglobulin-like domains. It is a multifunction transmembrane protein involved in synaptic plasticity, neurodevelopment and neurogenesis. NCAM appears on early embryonic cells and is important in the formation of cell collectives and their boundaries at sites of morphogenesis (1-3). It is preferentially expressed on human NK cells and a subset of T lymphocytes that mediate MHC-unrestricted cell-mediated cytotoxicity (1). Single nucleotide polymorphisms (SNPs) in the NCAM gene are reported to be significantly associated with bipolar disorder in the Japanese population (3).

Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:10,000
Immunohistochemistry: 1:500-1:2000
Immunocytochemistry: 1:100-1:250
Flow Cytometry: 1:150
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Manufactured incorporating RabMAb technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
100ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide corresponding to residues near the C-terminus of human NCAM/CD56.
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
Recognizes human CD56/NCAM at ~180kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lanier, L.L., et al., J. Immunol. 146(12): 4421-4426 (1991). 2. Cunningham, B.A., et al., Science 236(4803): 799-806 (1987). 3. Atz, M.E., et al., Psychiatr. Genet. 17(2): 55-67 (2007).