Technical Data
CD56 (NCAM-1, Neural Cell Adhesion Molecule)
The Immunoglobulin superfamily of cell adhesion molecules is related to the immunoglobulins proteins by structure. The family contains over a 100 members that include ICAM (intercellular adhesion molecule), NCAM (neural cell adhesion molecule) and NgCAM (neural-glial cell adhesion molecule). Both NCAM and NgCAM are calcium independent adhesion molecules. NCAM, the first CAM to be characterized, is expressed on all neurons from very early in development. It binds homophilically to NCAM on adjacent cells and has a variety of functions during neural development. One of these important functions is to hold developing neurites together during neuron outgrowth and formation of neural connections. The adhesive properties of NCAM are modulated during development and plasticity by the presence of the regulated carbohydrate, polysialic acid (PSA). The post-translational addition of alpha-2,8-linked PSA (NCAM-PSA) appears to contribute to synaptic plasticity during learning and memory formation and to the mechanisms of nerve regeneration.

Suitable for use in Western Blot, Immunofluorescence, Immunohistochemistry, ELISA and Functional Blocking. Other applications have not been tested.

Recommended Dilutions:
Western Blot (ECL): 1:1000 on 10ug of mouse brain lysate.
Immunohistochemistry (Wide range of fixatives): 1ug/ml will detect chicken NCAM; 2ug/ml will detect mouse NCAM.
ELISA: 0.3ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Neuraminidase-treated mouse brain membrane

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
50ug-20CBlue IceChickenRabbit
Highly purified chicken NCAM. Molecular Weight: 200-250kD; When treated with Neuraminidase, 140-180kD. A breakdown product of 160kD may also be visible.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS. No preservatives added.
Recognizes chicken Neural Cell Adhesion Molecule (NCAM). Species Crossreactivity: mouse. Species Sequence Homology: human and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Thiery, J.P., et al., J. Biol. Chem. 252: 6841-6845 (1977). 2. Hoffman, S., et al., J. Biol. Chem. 257: 7720-7729 (1982). 3. Hoffman, S., et al., PNAS USA 80(18): 5762-5766 (1983). 4. Cunningham, B.A., et al., Science 236: 799-806 (1987). 5. Rutishauser, A., et al., Science 240: 53-57 (1988). 6. Roth, J., et al., PNAS USA 85: 2999-3003 (1988). 7. Kibbelaar, R.E., et al., J. Pathol. 159: 23-28 (1989). 8. Tsen, G., et al., J. Biol. Chem. 270: 3392-3399 (1995). 9. Wang, X., et al., J. Neuroscience 20: 8551-8565 (2000). 10. Bleecker, J., et al., Neurology 58: 1779-1785 (2002). 11. Bouchard, J., et al., J. Neuroscience 24: 3040-3050 (2004). 12. Feng, Z., et al., Mol. Cell Biol. 22(15): 5357-5366 (2002). 13. Mosahebi, A., et al., Exp. Neurol. 173: 213-223 (2002). 14. Matsumoto-Miyai, K., et al., J. Neurosci. 23(21): 7727-7736 (2003). 15. Mikaelian, I., et al., Toxic. Pathol. 32: 181-191 (2004). 16. Pillai-Nair, N., et al., J. Neurosci. 25: 4659-4671 (2005).