		Technical Data
C2415-13B	CD62E (LECAM, E-Selectin, Endothelial Leukocyte Adhesion Molecule) (PE)

Description:
CD62E is expressed by cytokine-stimulated endothelial cells. It is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. This gene is present in single copy in the human genome and contains 14 exons spanning about 13kb of DNA. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis.

Applications:
Suitable for use in Studies in a wide range of cell interactions in the vasculature together with diagnostic implications, ELISA, Immunohistochemistry, Flow Cytometry and inhibits cellular adhesion. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 10ul/test.
Optimal dilution determined by the researcher.

Source:
Tissue culture supernatant

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Stable for 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

Type	Isotype	Clone	Grade
Mab	IgG1	2Q784	Affinity Purified

Size		Storage	Shipping	Source	Host
100 Tests		4°C Do Not Freeze	Blue Ice	Human	Mouse

Concentration:

Not Determined

Immunogen:

TNF-activated human umbilical vein endothelial cells

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, 10mM sodium azide, 1mg/ml BSA. Labeled with R-Phycoerythrin (PE).

Specificity:

Recognizes human CD62E expressed on activated endothelial cells. Species Crossreactivity: porcine.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Leucocyte Typing Workshop VI, Oxford University Press (1996) 2. Groves R.W. et al. Br. J. Dermatol. 124 117–123 (1991) 3. Bevilacqua, M.P. Nelson, R.M. J. Clin Invest 91, 379–87 (1993) 4. Leucocyte Typing Workshop V. Oxford University Press 2, 1501- 3 (1995) 5. Wellicome, S.M. Thornhill, M.H. Pitzalis, C. Thomas, D.S. Lanchbury, J.S.S. Panayi, G.S. et al. J. Immunol 144, 2558–65 (1990)